Squyres Lab

Publications

You can also see our work on Research Gate and Google Scholar.

All

2026

Extracellular matrix chemistry tunes bacterial biofilm metabolism and optimizes fitness
Extracellular matrix chemistry tunes bacterial biofilm metabolism and optimizes fitness
Jinyang Li, Georgia R. Squyres, Kathy Duong, Courtney Reichhardt, Matthew R. Parsek, Dianne K. Newman
Proceedings of the National Academy of Sciences  ·  06 Apr 2026  ·  doi:10.1073/pnas.2528666123
Starvation enhances bacterial survival through drying and rewetting at the single cell level
Starvation enhances bacterial survival through drying and rewetting at the single cell level
Jarek V. Kwiecinski, Georgia R. Squyres, Dani Or, Dianne K. Newman
openRxiv  ·  12 Jan 2026  ·  doi:10.64898/2026.01.12.697931

2025

Single-cell lysis patterns morphogenesis of eDNA in the matrix of i Pseudomonas aeruginosa i biofilms
Single-cell lysis patterns morphogenesis of eDNA in the matrix of Pseudomonas aeruginosa biofilms
Georgia R. Squyres, Dianne K. Newman
Proceedings of the National Academy of Sciences  ·  06 Oct 2025  ·  doi:10.1073/pnas.2514210122
Extracellular matrix chemistry tunes bacterial biofilm metabolism and optimizes fitness
Extracellular matrix chemistry tunes bacterial biofilm metabolism and optimizes fitness
Jinyang Li, Georgia R. Squyres, Kathy Duong, Courtney Reichhardt, Matthew R. Parsek, Dianne K. Newman
openRxiv  ·  18 Sep 2025  ·  doi:10.1101/2025.09.18.677115
SpoIIE drives asymmetric cell division in i B. subtilis i by sequential modulation of the cytokinesis machinery
SpoIIE drives asymmetric cell division in B. subtilis by sequential modulation of the cytokinesis machinery
Alexis Ryan, Georgia R. Squyres, Matthew J. Holmes, Alex Bisson, Ethan C. Garner, Niels Bradshaw
openRxiv  ·  10 Jun 2025  ·  doi:10.1101/2025.06.09.658746

2024

Biofilms as more than the sum of their parts: lessons from developmental biology
Biofilms as more than the sum of their parts: lessons from developmental biology
Georgia R Squyres, Dianne K Newman
Current Opinion in Microbiology  ·  01 Dec 2024  ·  doi:10.1016/j.mib.2024.102537
Real-time high-resolution microscopy reveals how single-cell lysis shapes biofilm matrix morphogenesis
Real-time high-resolution microscopy reveals how single-cell lysis shapes biofilm matrix morphogenesis
Georgia R. Squyres, Dianne K. Newman
openRxiv  ·  14 Oct 2024  ·  doi:10.1101/2024.10.13.618105
Cell constriction requires processive septal peptidoglycan synthase movement independent of FtsZ treadmilling in Staphylococcus aureus
Cell constriction requires processive septal peptidoglycan synthase movement independent of FtsZ treadmilling in Staphylococcus aureus
Simon Schäper, António D. Brito, Bruno M. Saraiva, Georgia R. Squyres, Matthew J. Holmes, Ethan C. Garner, Zach Hensel, Ricardo Henriques, Mariana G. Pinho
Nature Microbiology  ·  13 Mar 2024  ·  doi:10.1038/s41564-024-01629-6

2023

Processive movement of i Staphylococcus aureus i essential septal peptidoglycan synthases is independent of FtsZ treadmilling and drives cell constriction
Processive movement of Staphylococcus aureus essential septal peptidoglycan synthases is independent of FtsZ treadmilling and drives cell constriction
Simon Schäper, António D. Brito, Bruno M. Saraiva, Georgia R. Squyres, Matthew J. Holmes, Ethan C. Garner, Zach Hensel, Ricardo Henriques
openRxiv  ·  29 Jun 2023  ·  doi:10.1101/2023.06.29.547026
Mycobacterial nucleoid-associated protein Lsr2 is required for productive mycobacteriophage infection
Mycobacterial nucleoid-associated protein Lsr2 is required for productive mycobacteriophage infection
Charles L. Dulberger, Carlos A. Guerrero-Bustamante, Siân V. Owen, Sean Wilson, Michael G. Wuo, …, Michael Baym, Laura L. Kiessling, Ethan C. Garner, Eric J. Rubin, Graham F. Hatfull
Nature Microbiology  ·  23 Feb 2023  ·  doi:10.1038/s41564-023-01333-x

2021

Designing efficient genetic code expansion in Bacillus subtilis to gain biological insights
Designing efficient genetic code expansion in Bacillus subtilis to gain biological insights
Devon A. Stork, Georgia R. Squyres, Erkin Kuru, Katarzyna A. Gromek, Jonathan Rittichier, Aditya Jog, Briana M. Burton, George M. Church, Ethan C. Garner, Aditya M. Kunjapur
Nature Communications  ·  14 Sep 2021  ·  doi:10.1038/s41467-021-25691-4
Single-molecule imaging reveals that Z-ring condensation is essential for cell division in Bacillus subtilis
Single-molecule imaging reveals that Z-ring condensation is essential for cell division in Bacillus subtilis
Georgia R. Squyres, Matthew J. Holmes, Sarah R. Barger, Betheney R. Pennycook, Joel Ryan, Victoria T. Yan, Ethan C. Garner
Nature Microbiology  ·  18 Mar 2021  ·  doi:10.1038/s41564-021-00878-z
Treadmilling FtsZ polymers drive the directional movement of sPG-synthesis enzymes via a Brownian ratchet mechanism
Treadmilling FtsZ polymers drive the directional movement of sPG-synthesis enzymes via a Brownian ratchet mechanism
Joshua W. McCausland, Xinxing Yang, Georgia R. Squyres, Zhixin Lyu, Kevin E. Bruce, …, Bill Söderström, Ethan C. Garner, Malcolm E. Winkler, Jie Xiao, Jian Liu
Nature Communications  ·  27 Jan 2021  ·  doi:10.1038/s41467-020-20873-y

2020

Dynamics of bacterial cell division: Z ring condensation is essential for cytokinesis
Dynamics of bacterial cell division: Z ring condensation is essential for cytokinesis
Georgia R. Squyres, Matthew J. Holmes, Sarah R. Barger, Betheney R. Pennycook, Joel Ryan, Victoria T. Yan, Ethan C. Garner
openRxiv  ·  01 Jul 2020  ·  doi:10.1101/2020.06.30.180737
Erratum for the Report: Treadmilling by FtsZ filaments drives peptidoglycan synthesis and bacterial cell division by A. W. Bisson-Filho, Y.-P. Hsu, G. R. Squyres, E. Kuru, F. Wu, C. Jukes, Y. Sun, C. Dekker, S. Holden, M. S. VanNieuwenhze, Y. V. Brun, E. C. Garner
Erratum for the Report: “Treadmilling by FtsZ filaments drives peptidoglycan synthesis and bacterial cell division” by A. W. Bisson-Filho, Y.-P. Hsu, G. R. Squyres, E. Kuru, F. Wu, C. Jukes, Y. Sun, C. Dekker, S. Holden, M. S. VanNieuwenhze, Y. V. Brun, E. C. Garner
Science  ·  17 Jan 2020  ·  doi:10.1126/science.aba6311

2018

A central role for PBP2 in the activation of peptidoglycan polymerization by the bacterial cell elongation machinery
A central role for PBP2 in the activation of peptidoglycan polymerization by the bacterial cell elongation machinery
Patricia D. A. Rohs, Jackson Buss, Sue I. Sim, Georgia R. Squyres, Veerasak Srisuknimit, …, Andrew C. Kruse, Ethan C. Garner, Suzanne Walker, Daniel E. Kahne, Thomas G. Bernhardt
PLOS Genetics  ·  18 Oct 2018  ·  doi:10.1371/journal.pgen.1007726
An activation pathway governs cell wall polymerization by a bacterial morphogenic machine
An activation pathway governs cell wall polymerization by a bacterial morphogenic machine
Patricia D. A. Rohs, Jackson Buss, Sue Sim, Georgia Squyres, Veerasak Srisuknimit, …, Andrew C. Kruse, Ethan Garner, Suzanne Walker, Daniel Kahne, Thomas G. Bernhardt
openRxiv  ·  29 Jun 2018  ·  doi:10.1101/359208

2017

Treadmilling by FtsZ filaments drives peptidoglycan synthesis and bacterial cell division
Treadmilling by FtsZ filaments drives peptidoglycan synthesis and bacterial cell division
Alexandre W. Bisson-Filho, Yen-Pang Hsu, Georgia R. Squyres, Erkin Kuru, Fabai Wu, …, Cees Dekker, Seamus Holden, Michael S. VanNieuwenhze, Yves V. Brun, Ethan C. Garner
Science  ·  17 Feb 2017  ·  doi:10.1126/science.aak9973

2015

Facultative Control of Matrix Production Optimizes Competitive Fitness in Pseudomonas aeruginosa PA14 Biofilm Models
Facultative Control of Matrix Production Optimizes Competitive Fitness in Pseudomonas aeruginosa PA14 Biofilm Models
Jonas S. Madsen, Yu-Cheng Lin, Georgia R. Squyres, Alexa Price-Whelan, Ana de Santiago Torio, Angela Song, William C. Cornell, Søren J. Sørensen, Joao B. Xavier, Lars E. P. Dietrich
Applied and Environmental Microbiology  ·  15 Dec 2015  ·  doi:10.1128/AEM.02628-15